卢佳伟,李由然,石贵阳.聚乙烯亚胺改性聚丙烯腈中空膜固定脂肪酶[J].分子催化编辑部,2018,32(1):79-89
聚乙烯亚胺改性聚丙烯腈中空膜固定脂肪酶
The Modification of Polyacrylonitrile Hollow Membrane by Polyethyleneimine to Immobilize Lipase
投稿时间:2017-11-20  修订日期:2017-12-20
DOI:
中文关键词:  聚丙烯腈  表面改性  酶固定化  脂肪酶
英文关键词:polyacrylonitrile  surface modification  enzyme immobilization  lipase
基金项目:国家自然科学基金青年基金项目(No.31401674)(National Natural Science Foundation of China (No.31401674));"十三五"国家重点研发计划项目(No.2016YFD0401400)
作者单位E-mail
卢佳伟 江南大学 生物工程学院, 江苏 无锡 214122;江南大学 粮食发酵工艺与技术国家工程实验室, 江苏 无锡 214122  
李由然 江南大学 生物工程学院, 江苏 无锡 214122;江南大学 粮食发酵工艺与技术国家工程实验室, 江苏 无锡 214122  
石贵阳 江南大学 生物工程学院, 江苏 无锡 214122;江南大学 粮食发酵工艺与技术国家工程实验室, 江苏 无锡 214122 gyshi@jiangnan.edu.cn 
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中文摘要:
      聚丙烯腈是富腈基的高分子聚合物,易修饰改性,广泛应用于膜分离应用.我们以聚丙烯腈中空膜为载体,采用化学法交联聚乙烯亚胺并固定脂肪酶,固定过程中引入海藻酸钠,用CaCl2进行后处理,得到固定化脂肪酶PAN-PEI-SA/E-CaCl2载酶量为31.70(mg enzyme)/(g support),酶活为50.20 U/(g support),15次重复使用可保留58.77%的酶活,与游离酶相比耐酸性和耐温性有所提高,相同条件下与Nov 435相比,酶活更高,这表明最终得到的固定化脂肪酶有良好的工业应用前景.
英文摘要:
      Polyacrylonitrile is a nitrile-rich polymer and easy to be surface modified, and it has been widely applied in membrane separation. In this study, polyacrylonitrile hollow membrane was used as a support of enzyme immobilization by chemically cross-linking polyethyleneimine. Sodium alginate was added during immobilization, and the immobilized lipase was post-treated by CaCl2. The amount of loading enzyme of PAN-PEI-SA/E-CaCl2 was 31.70 (mg enzyme)/(g support) and the activity as 50.20 U/(g support).After 15 cycles of reactions, the immobilized enzyme retained 58.77% of initial enzyme activity, and showed an improvement of heat and acid resistance compared to free lipase. When it is compared to commercialized Nov 435 under same condition, PAN-PEI-SA/E-CaCl2 has higher enzyme activity during 15 cycles of reactions. It shows the immobilized lipase with PAN after surface modification has industrial application prospects.
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