| 岳磊,石贤爱.含离子液体介质中酶催化糖基化合成4-羟基苯乙基β-D-葡萄糖苷[J].分子催化,2011,(3):269-275 |
| 含离子液体介质中酶催化糖基化合成4-羟基苯乙基β-D-葡萄糖苷 |
| Enzymatic Glycosylation for Synthesis of 4-Hydroxyl Phenylethyl β-D-Glucoside in Medium Containing Ionic Liquids |
| 投稿时间：2011-03-29 修订日期：2011-05-12 |
| DOI： |
| 中文关键词: β-葡萄糖苷酶 4-羟基苯乙基β-D-葡萄糖苷 离子液体 糖基化反应 |
| 英文关键词:β-Glucosidase salidroside ionic liquid glycosylation |
| 基金项目:福建省发改委资助项目 |
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| 中文摘要: |
| 探讨了β-葡萄糖苷酶在离子液体/有机溶剂/缓冲溶液介质中催化直接糖基化反应合成4-羟基苯乙基β-D-葡萄糖苷。考察了反应体系组成、反应条件等因素对反应的影响。研究结果表明，1-丁基-3-甲基咪唑六氟磷酸盐（C4MIm•PF6）/1,4-二氧六环/柠檬酸-磷酸盐缓冲溶液是糖基化反应的合适介质。当三者的体积含量分别为17%、68%和15%，底物D-(+)-葡萄糖和4-羟基苯乙醇的含量分别为100 mg/mL和220 mg/mL、酶活力为12 U/mL时，在pH 6.0、50 ℃、275 rpm条件下进行直接糖基化，产物浓度可达18.9 mg/mL。 |
| 英文摘要: |
| The glycosylation catalyzed by β-glucosidase for synthesis of 4-hydroxyl phenylethyl β-D-glucoside (salidroside) in mixed medium of ionic liquids/organic solvent/buffer solution was studied. The effects of some important factors on the glycosylation such as the constitution of the medium, and the reaction conditions were investigated. The results showed that 1-butyl-3-methylimidazolium hexafluorophosphate (C4MIm•PF6) /1,4-dioxane/citric acid-phosphate buffer was the proper mixed medium for biosynthesis of salidroside. When the volume proportions of these components were 17%, 68% and 15% respectively, the product concentration would reach 18.9 mg/mL under these conditions: D-(+)-glucose 100 mg/mL, 4-hydroxyphenethyl alcohol (tyrosol) 220 mg/mL, β-glucosidase 12 U/mL, pH 6.0, 50 ℃ and 275 rpm for 120 h. |
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