| 张凡凡,夏仕文,谢永芳,刘姣,林晖.类产碱假单胞菌XW-40发酵-生物转化级联产生D-脯氨酸[J].分子催化,2021,35(4):328-334 |
| 类产碱假单胞菌XW-40发酵-生物转化级联产生D-脯氨酸 |
| Fermentation-biotransformation Cascade for the Production of D-proline by Pseudomonas pseudolcaligenes XW-40 |
| 投稿时间：2021-05-15 修订日期：2021-06-20 |
| DOI：10.16084/j.issn1001-3555.2021.04.003 |
| 中文关键词: 类产碱假单胞菌XW-40 发酵 生物转化 D-脯氨酸 脯氨酸脱氢酶 |
| 英文关键词:Pseudomonas pseudoalcaligenes XW-40 fermentation biotransformation D-proline proline dehydrogenase |
| 基金项目:重庆市研究生科研创新项目（CYS20270） |
|
| 摘要点击次数: 941 |
| 全文下载次数: 799 |
| 中文摘要: |
| 建立了一个新颖的用于产生D-脯氨酸的发酵-生物转化过程.发酵过程中，以DL-脯氨酸为发酵前体，类产碱假单胞菌XW-40利用L-对映体诱导产生脯氨酸脱氢酶，D-对映体完全保留.在最优条件下，发酵阶段产生6 g/L D-脯氨酸.生物转化过程中，细胞不经分离，发酵液直接作为反应介质.采用分批补料策略实现DL-脯氨酸中L-对映体的转化.DL-脯氨酸单批补料浓度为10 g/L，补料次数达到5批.通过发酵和生物转化的级联，累积的D-脯氨酸浓度达到31 g/L，ee>99%.推测了生物转化过程中D-脯氨酸产生的反应机理. |
| 英文摘要: |
| A novel fermentation-biotransformation cascade was developed for the production of D-proline. In the fermentation process with DL-proline as the precursor, the L-enantiomer was used to induce the production of proline dehydrogenase by Pseudomonas pseudoalcaligenes XW-40, while the D-enantiomer was completely retained. Under the optimal conditions, 6 g/L D-proline was produced in the fermentation stage. In the biotransformation process, the fermentation broth is directly used as the reaction medium without further separation of cells. The conversion of L-enantiomer in DL-proline was realized by fed-batch strategy. The feeding concentration of DL-proline in a single batch was 10 g/L, and the feeding times reached 5 batches. Through the cascade of fermentation and biotransformation, the D-proline was accumulated with 31 g/L and more than 99% enantiomeric excess. The mechanism of D-proline production in biotransformation was also postulated. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
