| 陈林林,张佳欣,李伟,王玲,宋佳琪,辛嘉英.无机杂化脂肪酶的制备及其催化性能研究[J].分子催化,2022,36(3):235-244 |
| 无机杂化脂肪酶的制备及其催化性能研究 |
| Preparation and Catalytic Properties of Inorganic Hybrid Lipase |
| 投稿时间：2022-03-01 修订日期：2022-03-31 |
| DOI：10.16084/j.issn1001-3555.2022.03.004 |
| 中文关键词: 脂肪酶 金属离子 杂化 催化活性 |
| 英文关键词:lipase metal ions hybrid catalytic activity |
| 基金项目:黑龙江省财政厅、中央支持地方高校改革发展资金人才培养支持计划项目(304017);哈尔滨市应用技术研究与开发项目(2014RFQXJ115) |
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| 中文摘要: |
| 采用脂肪酶为有机组分,Ca2+、Zn2+、Mn2+和Cu2+等4种金属磷酸盐为无机组分,制备脂肪酶-无机杂化纳米花,系统研究4种杂化脂肪酶的结构和催化活性.结果表明,制备的杂化脂肪酶具有花状的层级片状结构,并探究了无机杂化脂肪酶的最优反应时间为10 min,反应温度为40℃,pH值为8.4种金属离子制备的杂化脂肪酶包封率达到90%以上.相比于游离脂肪酶,杂化脂肪酶的催化水解活性、催化反应速率、耐温性、耐变性均有所提高,其中Ca2+金属磷酸盐合成的杂化脂肪酶催化效率是游离脂肪酶的1.87倍,合成的杂化脂肪酶表现出良好的重复使用性. |
| 英文摘要: |
| Lipase-inorganic hybrid nanoflowers were prepared by using lipase as the organic component and four metal phosphates including Ca2+, Zn2+, Mn2+ and Cu2+ as the inorganic component, and the structure and catalytic activity of the four hybrid lipases were systematically studied. The results show that the prepared hybrid lipase has a flower-like hierarchical lamellar structure, and the optimal catalytic conditions of inorganic hybrid lipase were as follows:recation time 10 min, reaction temperature 40℃, and pH=8. The encapsulation efficiency of the hybrid lipase reaches more than 90%. Compared with free lipase, the catalytic hydrolysis activity, the catalytic reaction rate, the temperature resistance, and the denaturant resistance of hybrid lipase were improved. The catalytic efficiency of hybrid lipase for Ca2+ metal phosphate synthesis is 1.87 times that of free lipase. The synthetic hybrid lipase showed good reusability. |
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