彭艳,李春霓,龙苋魏,李俊澄,陆群.使用响应面法优化酶动力学拆分(R/S)-δ-十四内酯[J].分子催化,2024,38(5):418-426 |
使用响应面法优化酶动力学拆分(R/S)-δ-十四内酯 |
Optimizing the Enzymatic Kinetic Resolution of (R/S)-δ-Tetradecalactone by Response Surface Methodology |
投稿时间:2024-06-07 修订日期:2024-08-27 |
DOI:10.16084/j.issn1001-3555.2024.05.003 |
中文关键词: 脂肪酶 特异性拆分 响应面法 羟基脂肪酸 |
英文关键词:lipase specific resolution response surface method hydroxyl fatty acids |
基金项目:国家自然科学基金资助项目(2019G010129) (The National Nature Science Foundation of China(2019G010129). |
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中文摘要: |
采用柱前衍生HPLC分析方法, 以δ-十四内酯为底物, 对一系列脂肪酶进行了筛选, 得到可特异性拆分底物为(R/S)-5-羟基十四烷酸的黑曲霉脂肪酶(ANL)和洋葱假单胞菌脂肪酶(PCL). 考察了pH、温度、助溶剂、酶酯比和反应时间对产物光学纯度(eep)、收率(Y)的影响. 通过响应面分析方法(CCD)确定了最佳反应条件, 优化后得到(S)-5-羟基十四烷酸(eep1≥ 99.9%, 收率为42.0%)和(R)-5-羟基十四烷酸(eep2≥ 99.9%, 收率达49.0%). 该研究符合绿色化学要求, 具有良好的应用潜力. |
英文摘要: |
A series of lipases were screened using δ-tetradecalactone as substrate by pre-column derivatization HPLC analysis. Aspergillus niger lipase (ANL) and Pseudomonas cepacia lipase (PCL) with specific hydrolysis substrate of (R/S)-5-hydroxytetradecanoic acid were obtained. The effects of pH, temperature, cosolvent, enzyme-ester ratio and reaction time on the optical purity (eep) and yield (Y) of the product were investigated. The optimum reaction conditions were determined by response surface analysis (CCD). After optimization, (S)-5-hydroxytetradecanoic acid (eep1 ≥ 99.9%, yield 42.0%) and (R)-5-hydroxytetradecanoic acid (eep2 ≥ 99.9%, yield 49.0%) were obtained. The system meets the requirements of green chemistry and has good application potential. |
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